Protein H2AFX map

Identifiers
H2A histone family, member X
HUGO:H2AFX , HGNC:4739, ENTREZ:3014, UNIPROT:P16104
HUGO:H2AFX HGNC:4739 ENTREZ:3014 UNIPROT:P16104 GENECARDS:H2AFX REACTOME:56152 KEGG:3014 ATLASONC:H2AFXID40783ch11q23 WIKI:H2AFX

Maps_Modules
HMC:SUSTAINING_PROLIFERATIVE_SIGNALLING
 Cell Cycle DNA Repair  map  / S_PHASE_CHECKPOINT  map
 Cell Cycle DNA Repair  map  / G2_M_CHECKPOINT  map
HMC:RESISTING_CELL_DEATH
 Regulated Cell Death  map  / DEATH_RECEPTOR_PATHWAYS  map
 Regulated Cell Death  map  / DNA_DAMAGE_RESPONSE  map
 Regulated Cell Death  map  / NECROPTOSIS  map
HMC:EVADING_GROWTH_SUPPRESSORS
 Survival  map  / WNT_CANONICAL  map

References
PMID:9488723, PMID:19772495, PMID:17303408
For S-phase checkpoint
PMID:15459660, PMID:12607003
synonym:H2AX

H2AFX|​S139_pho|​ubi@Nucleus

References
ce_re147( Cell Cycle DNA Repair  map ):
PMID:18006705
RNF8:MDC1 complex recruit HR-related proteins by this shifting from C_NHEJ to HR:
PMID:18001825
PMID:17303408
ce_re149:( Cell Cycle DNA Repair  map ) PMID:17303408, PMID:16377563
ce_re166( Cell Cycle DNA Repair  map ):
Fanconi recognition complex as FA core complex recruits NER, HR, TLS proteins that collectively repair the ICL. If Fanconi proteins participating in the recognition are dysactivated, there is no any other mechanism to recognize ILSs, therefore, even if NER, HR and TLS are correct, they will not perform the repair without core Fanconi proteins.
PMID:19686080

H2AFX|​S139_pho@Nucleus

References
ce_re90( Cell Cycle DNA Repair  map ):
PMID:9488723, PMID:19772495
PMID:17303408
ce_re92:( Cell Cycle DNA Repair  map ) PMID:16310392
ce_re147( Cell Cycle DNA Repair  map ):
PMID:18006705
RNF8:MDC1 complex recruit HR-related proteins by this shifting from C_NHEJ to HR:
PMID:18001825

H2AFX@Nucleus

References
ce_re92:( Cell Cycle DNA Repair  map ) PMID:16310392
ce_re90( Cell Cycle DNA Repair  map ):
PMID:9488723, PMID:19772495
PMID:17303408

H2AFX|​pho@Site of DNA damage

Maps_Modules
HMC:RESISTING_CELL_DEATH
 Regulated Cell Death  map  / DEATH_RECEPTOR_PATHWAYS  map
 Regulated Cell Death  map  / DNA_DAMAGE_RESPONSE  map
 Regulated Cell Death  map  / NECROPTOSIS  map

References
rc_re1488( Regulated Cell Death  map ):
influenceDelete: re to reactants
PMID:9488723
PMID:11571274
rc_re1479( Regulated Cell Death  map ):
PMID:16377563
PMID:16049003
rc_re1489( Regulated Cell Death  map ):
PMID:20118229
by PPM1D
PMID:20101220

H2AFX|​Y142_pho@Nucleus

Maps_Modules
HMC:RESISTING_CELL_DEATH
 Regulated Cell Death  map  / DEATH_RECEPTOR_PATHWAYS  map
 Regulated Cell Death  map  / DNA_DAMAGE_RESPONSE  map
 Regulated Cell Death  map  / NECROPTOSIS  map

References
rc_re1490( Regulated Cell Death  map ):
influenceDelete: reactants to re
PMID:19092802
by WICH complex (subunit identification by mass spectrometry: WSTF and SNF2H)
PMID:19234442
rc_re1494( Regulated Cell Death  map ):
PMID:16818236
upon UVA irradiation (no DSB), phosphorylation of H2AX S139 by JNK
effect of Y142 phosphorylation not assessed
interaction between JNK1 and H2AX Y142_pho dependent on FE65 (proposed FE65 scaffolding JNK to Y142_pho)
they propose H2AX Y142 phosphorylation as a switch toward apoptosis.
Even if not fully documented, the information from these 2 papers is merged into this representation where doubly phosphorylated H2AX cooperated with CAD for DNA fragmentation

H2AFX|​pho|​Y142_pho@Nucleus

Maps_Modules
HMC:RESISTING_CELL_DEATH
 Regulated Cell Death  map  / DEATH_RECEPTOR_PATHWAYS  map
 Regulated Cell Death  map  / DNA_DAMAGE_RESPONSE  map
 Regulated Cell Death  map  / NECROPTOSIS  map

References
rc_re1494( Regulated Cell Death  map ):
PMID:16818236
upon UVA irradiation (no DSB), phosphorylation of H2AX S139 by JNK
effect of Y142 phosphorylation not assessed
PMID:19234442
interaction between JNK1 and H2AX Y142_pho dependent on FE65 (proposed FE65 scaffolding JNK to Y142_pho)
they propose H2AX Y142 phosphorylation as a switch toward apoptosis.
Even if not fully documented, the information from these 2 papers is merged into this representation where doubly phosphorylated H2AX cooperated with CAD for DNA fragmentation
rc_re1044( Regulated Cell Death  map ):
PMID:12198487
by AIFM1
PMID:14716299
in vitro, by AIF+CypA
PMID:11452314
in DFFB KO MEFs by ENDOG

Modifications:
In compartment: Nucleus
  1. H2AFX@Nucleus map
  2. H2AFX|​S139_pho@Nucleus map
  3. H2AFX|​Y142_pho@Nucleus map
  4. H2AFX|​S139_pho|​ubi@Nucleus map
  5. H2AFX|​pho|​Y142_pho@Nucleus map
In compartment: Site of DNA damage
  1. H2AFX|​pho@Site of DNA damage map
Participates in complexes:
In compartment: Nucleus
  1. H2AFX:​PARP1@Nucleus map
  2. H2AFX|​S139_pho|​ubi:​MDC1|​pho@Nucleus map
  3. ATM|​S1981_pho|​S367_pho|​S1893_pho:​H2AFX|​S139_pho|​ubi:​MDC1|​pho:​MRE11*:​NBS1*|​S343_pho:​RAD50@Nucleus map
  4. BRCA1:​BRCA2:​FANCD2|​pho:​FANCI|​pho:​H2AFX|​S139_pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@Nucleus map
  5. BRCA1:​BRCA2:​FANCD2|​K561_ubi|​pho:​FANCI|​K523_ubi|​pho:​H2AFX|​S139_pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@Nucleus map
Participates in reactions:
As Reactant or Product:
  1. H2AFX|​S139_pho@Nucleus map → map H2AFX|​S139_pho|​ubi@Nucleus map
  2. H2AFX|​S139_pho|​ubi@Nucleus map + MDC1|​pho@Nucleus map → map H2AFX|​S139_pho|​ubi:​MDC1|​pho@Nucleus map
  3. FANCD2|​K561_ubi|​pho:​FANCI|​K523_ubi|​pho@Nucleus map + BRCA1|​S988_pho|​S1387_pho|​S1423_pho|​S1497_pho|​S1524_pho|​active@Nucleus map + BRCA2@Nucleus map + NBS1*|​S343_pho@Nucleus map + RAD51@Nucleus map + PCNA@Nucleus map + H2AFX|​S139_pho|​ubi@Nucleus map + TIP60*@Nucleus map → map BRCA1:​BRCA2:​FANCD2|​K561_ubi|​pho:​FANCI|​K523_ubi|​pho:​H2AFX|​S139_pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@Nucleus map
  4. BRCA1:​BRCA2:​FANCD2|​K561_ubi|​pho:​FANCI|​K523_ubi|​pho:​H2AFX|​S139_pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@Nucleus map → map BRCA1:​BRCA2:​FANCD2|​pho:​FANCI|​pho:​H2AFX|​S139_pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@Nucleus map
  5. H2AFX@Nucleus map + PARP1|​PolyADPribose_unk|​pho@Nucleus map → map H2AFX:​PARP1@Nucleus map
  6. H2AFX@Nucleus map → map H2AFX|​S139_pho@Nucleus map
  7. H2AFX|​S139_pho@Nucleus map → map H2AFX@Nucleus map
  8. ATM|​S1981_pho|​S367_pho|​S1893_pho:​MRE11*:​NBS1*|​S343_pho:​RAD50@Nucleus map + H2AFX|​S139_pho|​ubi:​MDC1|​pho@Nucleus map → map ATM|​S1981_pho|​S367_pho|​S1893_pho:​H2AFX|​S139_pho|​ubi:​MDC1|​pho:​MRE11*:​NBS1*|​S343_pho:​RAD50@Nucleus map
  9. H2AFX@Nucleus map → map H2AFX|​pho@Site of DNA damage map
  10. H2AFX|​pho@Site of DNA damage map → map H2AFX@Nucleus map
  11. H2AFX@Nucleus map → map H2AFX|​Y142_pho@Nucleus map
  12. H2AFX|​Y142_pho@Nucleus map → map H2AFX|​pho|​Y142_pho@Nucleus map
  13. H2AFX|​Y142_pho@Nucleus map → map H2AFX@Nucleus map
  14. H2AFX@Nucleus map → map H2A*@Nucleus map
As Catalyser:
  1. gHR_DNA_st1*@Nucleus map → map gHR_DNA_st2*@Nucleus map
  2. G1 phase@Nucleus map → map S phase@Nucleus map
  3. S phase@Nucleus map → map G2 phase@Nucleus map
  4. G2 phase@Nucleus map → map M phase@Nucleus map
  5. gHR_DNA_st2*@Nucleus map → map gHR_DNA_st3*@Nucleus map
  6. gFanconi_DNA_st4*@Nucleus map → map gHR_DNA_st2*@Nucleus map
  7. ATM@Nucleus map → map ATM|​S1981_pho|​S367_pho|​S1893_pho|​active@Nucleus map
  8. CHEK2@Nucleus map → map CHEK2|​pho|​active@Nucleus map
  9. Ku70*@Nucleus map + Ku80*@Nucleus map → map Ku70*:​Ku80*@Nucleus map
  10. DNA-PK*@Nucleus map → map DNA-PK*|​pho@Nucleus map
  11. DNA~integrity@Nucleus map → map DNA~fragmentation@Nucleus map
  12. MDC1|​Nter-SDT-motifs_pho@Nucleus map → map MDC1|​Nter-SDT-motifs_pho@Site of DNA damage map