Identifiers
NAME:SMAD2:SMAD3:SMAD4:SP1
Maps_Modules
HMC:ACTIVATING_INVASION_AND_METASTASIS
EMT Senescence / EMT_REGULATORS
References
PMID:11013220
Cooperation and physical interaction of Smad2, Smad3, Smad4 with SP1 at the promoter of CDKN2B (p15INK4B) gene
This interaction provides a mechanism underlying the TGFB-induced growth arrest
em_emtc_emtc_re281( EMT Senescence ):
PMID:11283614
Miz1: a Myc-interacting Zinc-finger protein that recognizes certain transcriptional initiator elements
One of this transcriptional initiator element contains the sequence 5'-CCCACTCTGC corresponding to the p15INK4B transcriptional intiator
Physical interaction between Smad proteins and Miz-1:
-Immunoprecipitation of endogenous Smad2 and Smad3 followed by anti-Miz western bloting provided evidence for an association
-This association was stimulated by addition of TGFB
-Interaction was observed between Miz1 and Smad3 and Smad4 but no clear interaction was observed between Miz1 and Smad2
-Smad3 and weakly Smad4 can directly and selectively bind to Miz1 through the MH1 domain
-Although Sp1-Smad interactions have been reported, Sp1 does not directly bind to Miz1, even in the presence of TGFB.
-The Smad-interaction region of Miz1 does not overlap with the Myc-interaction region.
-Myc did not inhibit the Smad-Miz1 interaction
-Miz1 can bridge Smad proteins and Myc when these proteins coexist.
+ (SMAD2|S467_pho|S464_pho|S465_pho:SMAD3|S422_pho|S423_pho|S425_pho:SMAD4:SP1)|active@Nucleus → (MIZ1*:SMAD2|S467_pho|S464_pho|S465_pho:SMAD3|S422_pho|S423_pho|S425_pho:SMAD4:SP1)|active@Nucleus