Identifiers
NAME:ELC_MYOSIN_II*:MYH*:RLC_MYOSIN_II*
Maps_Modules
HMC:TUMOR_PROMOTING_INFLAMMATION
HMC:ACTIVATING_INVASION_AND_METASTASIS
Cancer Associated Fibroblasts / MOTILITY
References
CASCADE:TGFB
CASCADE:LIF
PMID:18037882
Rho???ROCK function is required in leading fibroblasts probably downstream of integrins a5b1 and a3b1
Actomyosin contractility in CAFs, as in many other cell types, results from phosphorylation of MLC2 in myosin II downstream of the Rho kinases ROCK I and II
PMID:24857661
Long-term stimulation of both TGF-?? and LIF cytokines also upregulated RhoA small GTPase and myosin light chain 2 (MLC2) proteins, leading to an increase in MLC2 phosphorylation at ser19, which attests for an increased activity (Figure S2D). Finally, forced expression of an active form of ROCK (ROCK-ER) (Croft and Olson, 2006) following 4-hydroxytamoxifen (4OHT) treatment was sufficient to induce hDF contractility (Figure S2E), proinvasive capacity (Figures S2Fa and b), and MLC2 phosphorylation (Figure S2G) and also rescued the inhibitory effect of P6 or anti-LIF antibody treatments under TGF-??1 stimulation
Identifiers
NAME:ELC_MYOSIN_II*:MYH*:RLC_MYOSIN_II*
Maps_Modules
HMC:TUMOR_PROMOTING_INFLAMMATION
HMC:ACTIVATING_INVASION_AND_METASTASIS
Cancer Associated Fibroblasts / MOTILITY
References
CASCADE:LIF
CASCADE:TGFB
PMID:12194823
Rho-Kinase and Myosin-II Control Phagocytic Cup Formation during CR3, but Not Fc??R, Phagocytosis
inhibition of the Rho ??? ROK ??? myosin-II pathway caused a decreased accumulation of Arp2/3 complex and F-actin around bound particles, which led to a reduction in CR-mediated phagocytic engulfment.
+ RLC_MYOSIN_II*@Cytosol + MYH*|hm2@Cytosol → ELC_MYOSIN_II*:MYH*|hm2:RLC_MYOSIN_II*@Cytosol
→ ELC_MYOSIN_II*:MYH*|hm2:RLC_MYOSIN_II*|pho@Cytosol
→ Actomyosin_contractility@default
→ ELC_MYOSIN_II*:MYH*|hm2:RLC_MYOSIN_II*@Cytosol