Identifiers
HUGO:EP300
Maps_Modules
HMC:TUMOR_PROMOTING_INFLAMMATION
HMC:ACTIVATING_INVASION_AND_METASTASIS
Cancer Associated Fibroblasts / CORE
References
CASCADE:TGFB
PMID:17559085
in both skin and lung fibroblasts, TGF-?? enhanced p300 mRNA, and protein expression.
The human p300 gene has been recently cloned. The regulatory region is highly GC-rich, and contains six early growth response 1 (Egr-1) binding sites, along with serum response elements and Sp1 binding sites (Yu et al., 2004).
Ectopic expression of Egr-1 in skin fibroblasts by itself stimulated basal p300 gene expression, and further enhanced the stimulation induced by TGF-??. Furthermore, reporter constructs driven by p300 promoter fragments harboring mutations in the Egr-1 binding sites failed to respond to TGF-?? in transient transfection assays (Ghosh et al., unpublished data). These observations establish a critical role for Egr-1 in the regulation of p300 gene expression by TGF-?? in normal fibroblasts.