Identifiers
protein kinase C, gamma
HUGO:PRKCG, HGNC:9402, ENTREZ:5582, UNIPROT:P05129, GENECARDS:GC19P054382 ??
Maps_Modules
HMC:ACTIVATING_INVASION_AND_METASTASIS
EMT Senescence / EMT_REGULATORS
EMT Senescence / CELL_CELL_ADHESIONS
EMT Senescence / GAP_JUNCTIONS
References
GJA1 is so-called Connexin 43
PMID:16492141
Binding partners proteins of Connexin 43 (GJA1):
-Kinases: v-Src, c-Src, PKC, PKA, MAPK, Casein kinase 1, Cdc2 kinase
-TIGHT_JUNCTIONS scaffold proteins: ZO1, ZO2, caveolin 1
-Cytoskeleton: b-catenin, a-tubulin, b-tubulin
-Others: Drebrin, NOV, CIP85
PMID:10871288
Interaction between Connexin 43 (GJA1) and PKC (alpha, betat and gamma subunits)
The 3 subunits phosphorylate GJA1 at Ser368 and reduce Gap junctions activity.
PMID:10679481
Fibroblast growth factor-2 (FGF-2)decreases cardiomyocyte GJ permeability by stimulating phosphorylation of connexin-43
FGF-2 activates receptors linked to PKC and MAPK
In immunoprecipitation experiments using specific anti-Cx43 antibodies, PKCE but not PKCA coprecipitated with Cx43.
FGF-2 increased levels of coprecipitated PKCE, suggesting increased association between PKCE and Cx43 on stimulation.
To conclude, PKC mediates the FGF-2???induced effects on cardiac GJs and that PKC likely interacts with and phosphorylates cardiac Cx43 at sites of intercellular contact