References
PMID:17575049
Positive feedback between Dia1 (so-called DIAPH1), LARG (so-called ARHGEF12), and RhoA regulates cell morphology and invasion.
The FH2 domain of Dia1 stimulates the guanine nucleotide exchange activity of LARG in vitro.
Dia1 is necessary for LPA-stimulated Rho-ROCK signaling and bleb-associated cancer cell invasion.
Thus, Dia1-dependent RhoA activation constitutes a positive feedback mechanism to modulate cell behavior.
Dia1 is required and sufficient for full LPA- induced activation of RhoA and downstream ROCK signaling. This effect can be mediated through interaction of Dia1 with LARG
Dia1, in addition to its role as a downstream RhoA effector, can function upstream of RhoA.