References
PMID:14744429
RhoA is the prototypical member of the Rho GTPase family, which regulates many cellular processes, including cellular adhesion, motility, and polarity
RhoA is an important modulator of cell junction formation and stability:
PMID:7479854
Rho protein regulates tight junctions and perjunctional actin organization in polarized epithelia
PMID:9362522
Rho subfamily is necessary for the formation of both the cadherin-based cell???cell adhesion and the tight junction
PMID:9660866
RhoA and Rac1 regulate gate and fence functions of the TJ, and play a role in the spatial organization of TJ proteins at the apex of the lateral membrane
PMID:12361600
PMID:11992112
RhoA functions to maintain apical-basal polarity and cell junctions.
PMID:11739775
PMID:11729192
RhoA can stabilize tight junctions and increase transepithelial resistance.
PMID:10579713
Human ECT2 (so-called ARHGEF31)is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis.
PMID:10837491
The level of GTP-Rho did not change significantly from the S phase to the prometaphase, but increased thereafter, peaking in the telophase, and returned to the original level in the G1 phase.
Expression of the dominant negative form of ECT2 completely suppressed both the rise of GTP-Rho in the telophase and the increased GDP-GTP exchange activity in the mitotic cell extracts.
These results suggest a critical role of ECT2 in Rho activation during cytokinesis
PMID:11724822
Insulin-like growth factor (IGF)-1 plays crucial roles in growth control and rearrangements of the cytoskeleton
LARG is an Rho guanine nucleotide exchange factor, which contains a PDZ domain responsible for IGF-1 binding.
LARG induced the formation of stress fibers in NIH 3T3 fibroblasts.
MDCKII epithelial cells treated with IGF-1 results in activated Rho and its effector Rho-associated kinase and actin stress fibers were enhanced.
IGF-1 activates the Rho pathway via a LARG/IGF-1 receptor complex and thereby regulates cytoskeletal rearrangements.