Boolean_logic_gate_and

1. SNAI1@Nucleus and TWIST1|​S68_pho@Nucleus

Inhibition

1. (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Nucleus

Modulation

1. Angiogenesis@Nucleus

Physical_stimulation

1. TWIST1|​S68_pho@Nucleus
2. SMAD2_3*:​SMAD4@Nucleus
3. HMGA2:​SMAD2_3*:​SMAD4@Nucleus
4. SNAI1@Nucleus

References
PMID:20731704
PMID:19208835
TCF8 (ZEB1) is up-regulated in endothelial cells during angiogenesis, acting as a negative regulator.
PMID:21317430
In NMuMG cells treated with TGFB1 Snail1 RNA and protein are induced 1 h after addition of the cytokine preceding Zeb1 up-regulation that requires 6???8 h.
Zeb1 gene expression is caused by increased RNA levels but also by enhanced protein stability and is markedly dependent on Snail1 because depletion of this protein prevents Zeb1 protein and RNA up-regulation
Snail1 controls Zeb1 expression at multiple levels and acts cooperatively with Twist in the ZEB1 gene transcription induction
PMID:16831886
PMID:18832382
HMGA2 directly binds to the SNAIL1 promoter and acts as a transcriptional regulator of SNAIL1 expression.
HMGA2 cooperates with SMAD3 and SMAD4 to execute a dramatic super-induction of the SNAIL1 promoter.
Same results were obtained with SNAI2, ZEB1, ZEB2 and TWIST1
HMGA2 cooperates with TGFB1 signaling to represse ID2 transcriptomal expression